Global Post Translational Modification (PTM) Workflows
Next-Generation PTM Services
Our workflows include the following PTM's
- Phosphorylation
- Ubiquitylation
- Acetylation
- Methylation
- Hydroxylation
- Glycosylation
- Others on request, please contact
SysQuant delivers actionable knowledge on key biological processes driving disease and response to treatment
Precision Medicine could transform treatment outcomes but requires deeper understanding of disease biology, drug-target interaction and a system-wide view of mechanisms of action and resistance. For example global phosphoproteomic profiling of tissues and cells can identify new targets and candidate biomarkers for early diagnosis, drug selection and monitoring of treatment outcomes. Combining 11-, 18- or 35-plex labeling with the fastest, most accurate mass spectrometers, SysQuant® provides the most comprehensive analysis of protein activity across regulatory and signaling pathways.
| Applications | Sample Types | Species | Analysis Method |
|---|---|---|---|
| Pre-Clinical Drug Development | Cultured cells, Tumor Xenografts (fresh/ frozen) | All Mammals, Drosophila & Zebrafish | Mechanism of Action Mechanism of Resistance Toxicity Profiling Pharmacodynamics Biomarker Discovery |
| Clinical Drug Development | Tumor biopsy, Patient-Derived Xenograft, Patient Derived Cell Culture (fresh/frozen) | Human | Mechanism of Action Mechanism of Resistance Pharmacodynamics Biomarker Validation |
| Clinical Medicine | Tumor Biopsy, Patient-Derived Xenograft, Patient Derived Cell Culture (fresh/frozen) | Human | Optimized Drug Selection Outcome Monitoring Intelligent Drug Switching |
The SysQuant Global Phosphoproteomic Workflow Offers:
- Unbiased survey of cell signaling pathways regulated by PTM's such as phosphorylation
- Combined power of TMT 11 and 18-plex reagents and Orbitrap Tribrid mass spectrometry
- ~1,000,000 quantitative data points per TMT 11-, 18- or 35-plex experiment
- >35,000 proteins and ~40,000 phosphorylation sites*
- >8,000 unique protein groups and >12,000 unique phosphorylation sites*
- Standardized data outputs in Excel and CSV files
- Automated annotation of biological features from GO terms, KEGG, Phosphosite Plus, UniProt & Drugbank
- Common protocol for all sample types
- Results in 6-8 weeks (based on up to a 34 sample study as 2 x 18-plex experiments)
- Optional statistical analysis and biological interpretation packages
*The difference between the number of proteins and unique protein groups reflects redundancy in peptide sequences between protein forms. A peptide that is common to the eight isoforms of tau will be counted 8 times for the number of proteins but only once for the number of unique protein groups. It is now widely considered that typical cells/tissues express between 8 and 10,000 protein groups.